花药预处理对玉米游离小孢子存活率的影响*
陆瑞菊 王亦菲 孙月芳 周润梅 黄剑华 |
花药预处理对玉米游离小孢子存活率的影响* |
实验生物学报 |
2005 |
38(3): 265-271 |
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| 摘要：摘要：以大田种植的甜玉米“申甜1号”和糯玉米“沪玉糯1号”的花药为供试材料，比较了预处理液的渗透压（甘露醇浓度）、添加不同浓度秋水仙碱、脯氨酸和预处理温度对游离小孢子存活率的影响。结果表明：由低到高的渗透压变化处理(0.35mol/L 24h → 0.40mol/L 24h → 0.45mol/L 24h，添加秋水仙碱（200mg/L）、脯氨酸（200mg/L）和15℃、48h的预处理分别能明显地提高游离小孢子的存活率。对上述处理的可能作用机理进行了推测。 关键词：玉米 小孢子存活率 秋水仙碱 脯氨酸 甘露醇 EFFECTS OF ANTHER - PRETREATMENTS ON THE SURVIVAL PERCENTAGE OF ISOLATED MICROSPORES OF MAIZE LU RuiJu WANG YiFei SUN YueFang ZHOU RunMei HUANG JianHua (Shanghai Key Lab. of Agricultural Genetics and Breeding; Agro-Biotech Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106) Abstract Using maize anthers of 2 cultivars maize as experimental materials, the effects of mannitol, colchcine and proline concentration and pretreating temperature in pretreatment solution on the survival percentage of isolated microspore were compared. The survival percentage of isolated microspores were able to be respectively and obviously improved by pretreating anthers with mannitol from low to high concentration (0.35 mol/L, 24h→0.40 mol/L, 24h→0.45 mol/L, 24h), or with colchicine 200 mg/L or with proline 200 mg/L and pretreating at 15℃ for 48h. The probable mechanism of pretreatments was discussed. Key words: Zea mays L., the survival microspore percentage, colchicine, proline, mannitol |
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